SQS2 Sample Preparation
Boar semen samples to be analysedin the SQS2 must be previously stained with the SQStest by following the next steps:
1. Add 1 part of semen to 9 parts of extender and mix them to make a 1:9 dilution.
2. Transfer 5 μl of the diluted sperm sample to the fluorochrome tube and mix gently until its homogenization; the mixture should be colored in red.
3. Finally, fill-in one area of the slide with the stained sample.